Cytokeratin 8/18 Antibody, IgG1, Clone: [K8.8 + DC10], Mouse, Monoclonal

Produktübersicht

• Artikelname: Cytokeratin 8/18 Antibody, IgG1, Clone: [K8.8 + DC10], Mouse, Monoclonal
• Artikelnummer: NBT-MSM6-691-P1ABX
• Hersteller Artikelnummer: MSM6-691-P1ABX
• Alternativnummer: NBT-MSM6-691-P1ABX-100
• Hersteller: NeoBiotechnologies
• Wirt: Mouse
• Kategorie: Antikörper
• Applikation: FC, IF, IHC, WB
• Spezies Reaktivität: Human
• Immunogen: Keratin preparation from a human carcinoma (K8.8), PMC-42 human breast carcinoma cells (DC10)
• Alternative Synonym: CARD2, CK8, CYK8, CYKER, Cytokeratin Endo A, DreK8, EndoA, K2C8, K8, Keratin 8, Krt 2.8, KRT8, Type-II Keratin Kb8. Cell Proliferation-inducing Gene 46 Protein, CK18, CYK18Cytokeratin Endo B, K18, Keratin-18, Kerd, KRT18

Produktbeschreibung

Cytokeratin 8 (CK8) belongs to the type II (or B or basic) subfamily of high molecular weight cytokeratins and exists in combination with cytokeratin 18 (CK18). This MAb cocktail recognizes all simple epithelia including glandular epithelium, for example thyroid, female breast, gastrointestinal tract, respiratory tract, and urogenital tract including transitional epithelium. All adenocarcinomas and most squamous carcinomas are positive but keratinizing squamous carcinomas are usually negative. This antibody is useful in demonstrating the presence of Paget cells, there is very little keratin 18 in the normal epidermis so only Paget cells are stained.Immunohistochemical staining with this MAb is indistinguishable from that obtained with monoclonal antibody 5D3.

Produkteigenschaften

• Klonalität: Monoclonal
• Klon-Bezeichnung: [K8.8 + DC10]
• Molekulargewicht: 52.5kDa (CK8), 45kDa (CK18)
• Isotyp: IgG1
• NCBI: 3856
• UniProt: P05787
• Formulierung: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Antibody Type: Monoclonal Antibody

Anwendungsbeschreibung

• Anwendungsbeschreibung: Flow Cytometry (1-2ug/million cells), Immunofluorescence (1-2ug/ml), Western Blot (1-2ug/ml), Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA

Bilder

SDS-PAGE Anal

Formalin-fixed, paraffin-embedded human Ovarian Cancer stained with Cytokeratin 8/18 Monoclonal Antibody (K8.8 + DC10).

Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with Cytokeratin 8/18 Monoclonal Antibody (K8.8 + DC10).

SDS-PAGE Analysis of Purified Cytokeratin 8/18 Monoclonal Antibody (K8.8 + DC10). Confirmation of Purity and Integrity of Antibody.