Mouse anti Human CD5, conjugated with FITC, IgG2a, Clone: [M28623], Monoclonal

Produktübersicht

• Artikelname: Mouse anti Human CD5, conjugated with FITC, IgG2a, Clone: [M28623], Monoclonal
• Artikelnummer: NMB-0052
• Hersteller Artikelnummer: 0052
• Alternativnummer: NMB-0052
• Hersteller: NordicMubio
• Wirt: Mouse
• Kategorie: Antikörper
• Applikation: FC
• Spezies Reaktivität: Human
• Immunogen: CD5=Derived from the hybridization of mouse NS-1/Ag4 myeloma cells with spleen cells of BALB/c mice immunized with human t-acute lymphoblastic leukemia (ALL) cells.
• Konjugation: FITC
• Alternative Synonym: T-cell surface glycoprotein CD5, Lymphocyte antigen T1/Leu-1

Produktbeschreibung

Identification of human helper/inducer T cells expressing the 67,000 M.W. surface antigen, 85% peripheral blood lyymphocytes that form rosettes with sheep red blood cells (E+), and a small subset of B cells.

Produkteigenschaften

• Klonalität: Monoclonal
• Konzentration: Titered for flow cytometry
• Klon-Bezeichnung: [M28623]
• Isotyp: IgG2a
• UniProt: P06127
• Puffer: Provided as solution in phosphate buffered saline with 0.08% sodium azide and 0.2% carrier protein
• Reinheit: Protein A/G Chromatography
• Formulierung: FITC
• Formel: Provided as solution in phosphate buffered saline with 0.08% sodium azide and 0.2% carrier protein

Anwendungsbeschreibung

• Anwendungsbeschreibung: PBMC: Add10 µl of MAB/106 PBMC in 100 µl PBS. Mix gently and incubate for 15 minutes at 2o to 8oC. Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. WHOLE BLOOD: Add10 µl of MAB/100 µl of whole blood. Mix gently and incubate for 15 minutes at room temperature 20oC. Lyse the whole blood. Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. See instrument manufacturers instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope.