Mouse anti Human CD8 FITC - CD28 PE, FITC/RPE

Produktübersicht

• Artikelname: Mouse anti Human CD8 FITC - CD28 PE, FITC/RPE
• Artikelnummer: NMB-0828
• Hersteller Artikelnummer: 0828
• Alternativnummer: NMB-0828
• Hersteller: NordicMubio
• Wirt: Mouse
• Kategorie: Antikörper
• Applikation: FC
• Spezies Reaktivität: Human
• Immunogen: CD8=Derived from the hybridization of mouse NS-1 myeloma cells with spleen cells from BALB/c mice immunized with human perherial blood T lymphocytes.CD28=Derived from the hybridization of mouse Sp2/O-Ag14 myeloma cells with spleen cells of BALB/c mice immunized with the HPB-ALL T-cell line.
• Konjugation: FITC/RPE
• Alternative Synonym: CD8 FITC - CD28 PE

Produktbeschreibung

Identification of human cytotoxic/suppressor T cells expressing the 32 and 43,000 M.W. surface antigen. CD38 antigen is an integral membrane glycoprotein, M.W. 45 kD. Anti-human CD28 binds the 44kDa MW cell surface protein on the surface of most T cells.

Produkteigenschaften

• Konzentration: Titered for flow cytometry
• Isotyp: IgG1 (F)/IgG1 (PE)
• UniProt: P01732
• Puffer: Provided as solution in phosphate buffered saline with 0.08% sodium azide and 0.2% carrier protein
• Reinheit: Protein A/G Chromatography
• Formulierung: Bi-Test (FITC/RPE) Reagent
• Formel: Provided as solution in phosphate buffered saline with 0.08% sodium azide and 0.2% carrier protein

Anwendungsbeschreibung

• Anwendungsbeschreibung: PBMC: Add 10 µl of MAB/10 6 PBMC in 100 µl PBS. Mix gently and incubate for 15 minutes at 2 to 8C. Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. WHOLE BLOOD: Add 10 µl of MAB/100 µl of whole blood. Mix gently and incubate for 15 minutes at room temperature (20 C). Lyse the whole blood. Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. See instrument manufacturers instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope. Storage: Antibodies are supplied in PBS, 0.08% sodium azide and 0.2% protein carrier for FITC and PE. Antibodies should be stored at 4-8oC. Mabs should not be frozen. Reagents are stable for the period shown on the vial label when stored properly.