Mouse anti Human CD3 FITC - CD4 PE, FITC/RPE

Produktübersicht

• Artikelname: Mouse anti Human CD3 FITC - CD4 PE, FITC/RPE
• Artikelnummer: NMB-B034S
• Hersteller Artikelnummer: B034S
• Alternativnummer: NMB-B034S
• Hersteller: NordicMubio
• Wirt: Mouse
• Kategorie: Antikörper
• Applikation: FC
• Spezies Reaktivität: Human
• Immunogen: CD3=Derived from the hybridization of mouse NS-1 myeloma celss with spleen cells from BALB/c mice immunized with human thymocytes. CD4=Derived from the hybridization of mouse NS-1 myeloma cells with spleen cells from BALB/c mice immunized with human perherial blood T lymphocytes.
• Konjugation: FITC/RPE
• Alternative Synonym: CD3 FITC - CD4 PE

Produktbeschreibung

The CD3 epitope is expressed on the epsilon chain of the CD3/T cell antigen receptor (TcR) complex. CD3 is present on 65-85% of thymocytes and has a mitogenic effect on peripheral blood T cells. Identification of human T cells expressing the 22-28,000 M.

Produkteigenschaften

• Konzentration: Titered for flow cytometry
• UniProt: P07766
• Puffer: Provided as solution in phosphate buffered saline with 0.08% sodium azide and 0.2% carrier protein
• Reinheit: Protein A/G Chromatography
• Formulierung: Bi-Test (FITC/RPE) Reagent
• Formel: Provided as solution in phosphate buffered saline with 0.08% sodium azide and 0.2% carrier protein

Anwendungsbeschreibung

• Anwendungsbeschreibung: PBMC: Add10 µl of MAB/10 6 PBMC in 100 µl PBS. Mix gently and incubate for 15 minutes at 2 to 8C. Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. WHOLE BLOOD: Add10 µl of MAB/100 µl of whole blood. Mix gently and incubate for 15 minutes at room temperature 20C. Lyse the whole blood. Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. See instrument manufacturers instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope.