As a fluorescent marker for biotine conjugates. It is of critical importance always to establish by titration the optimum working dilution, since it depends on the variables of the test arrangement. Working dilutions are usually from 2 to 5 µg/ml.
ELISA,Immunocytochemistry,Immunohistochemistry (frozen),(In)direct immunofluorescence. The conjugate is shipped at ambient temperature and must be stored at 4C. Reconstitute the lyophilized product by adding 0.1 ml sterile water. Spin down to remove insoluble particles, divide into small aliquots, and store as a liquid at or below -20C by adding an equal volume of glycerol. Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the conjugate. Dilutions should be stored at +4C, not refrozen, and preferably used the same day. Avoid exposure to light.
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