A portion of amino acids 328-362 from human NEFH was used as the immunogen for the NF-H antibody.
Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. NF-H has an important function in mature axons that is not subserved by the two smaller NF proteins.
The stated application concentrations are suggested starting points. Titration of the NF-H antibody may be required due to differences in protocols and secondary/substrate sensitivity.
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