A portion of amino acids 135-163 from the human protein were used as the immunogen for the ATP5B antibody.
ATP5B is a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel consists of three main subunits (a, b, c). It is the beta subunit of the catalytic core.
Western blot: 1:1000,Immunohistochemistry (FFPE): 1:25-1:100,Flow cytometry: 1:25 (1x10e6 cells),Immunofluorescence: 1:25
Anwendungsbeschreibung:
The stated application concentrations are suggested starting points. Titration of the ATP5B antibody may be required due to differences in protocols and secondary/substrate sensitivity.
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