E.coli-derived human TGM5 recombinant protein (Position: E146-K608) was used as the immunogen for the TGM5 antibody.
TGM5 antibody detects Transglutaminase-5, encoded by the TGM5 gene on chromosome 15q15.2. TGM5 antibody is used in research on epidermal biology, keratinocyte differentiation, and skin barrier formation. TGM5 belongs to the transglutaminase family of Ca2+-dependent enzymes that catalyze crosslinking of proteins via isopeptide bonds. TGM5 is expressed primarily in epidermis and other stratified epithelia, where it contributes to cornified envelope formation, strengthening skin barrier integrity. Mutations in TGM5 cause acral peeling skin syndrome, highlighting its essential role.Structurally, TGM5 is a ~78 kDa enzyme with an N-terminal beta-sandwich domain, a catalytic core containing the conserved transglutaminase triad, and two C-terminal beta-barrel domains. The catalytic cysteine forms thioester intermediates during transamidation reactions. Structural similarities to other transglutaminases confirm conserved mechanisms but tissue-specific regulation.Functionally, TGM5 catalyzes crosslinking of structural proteins including involucrin, loricrin, and small proline-rich proteins. This activity stabilizes the cornified envelope of keratinocytes, ensuring epidermal resilience and barrier function. Knockdown or mutation disrupts barrier integrity, leading to peeling and sensitivity. Researchers employ TGM5 antibody to study keratinocyte biology, epidermal differentiation, and barrier formation.Clinically, TGM5 mutations cause acral peeling skin syndrome, characterized by painless shedding of the outer epidermis. Pathogenic variants impair catalytic activity, disrupting keratinocyte crosslinking. TGM5 is also studied in cancer, where altered expression influences differentiation and tumor progression. It may serve as a biomarker of keratinocyte differentiation status in skin diseases. NSJ Bioreagents supplies TGM5 antibody as a reagent for dermatology and barrier research.Experimentally, TGM5 antibody is applied in western blotting to detect the ~78 kDa protein, in immunohistochemistry to study epidermal localization, and in immunofluorescence to visualize keratinocyte differentiation. Enzyme activity assays combined with TGM5 antibody confirm correlations between expression and functional crosslinking activity.
