An E.coli-derived zebrafish NF45/ILF2 recombinant protein (amino acids Y23-K356) was used as the immunogen for the Zebrafish ILF2 antibody.
Interleukin enhancer-binding factor 2, also called Nuclear factor of activated T-cells 45 kDa, is a protein that in humans is encoded by the ILF2 gene. The protein encoded by this gene is a transcription factor required for T-cell expression of the interleukin 2 gene. It also binds RNA and is an essential component for encapsidation and protein priming of hepatitis B viral polymerase. The encoded 45 kDa protein (NF45, ILF2) forms a complex with the 90 kDa interleukin enhancer-binding factor 3 (NF90, ILF3), and this complex has been shown to affect the redistribution of nuclear mRNA to the cytoplasm, to repair DNA breaks by nonhomologous end joining, and to negatively regulate the microRNA processing pathway. Knockdown of NF45 or NF90 protein retards cell growth, possibly by inhibition of mRNA stabilization. Alternative splicing results in multiple transcript variants. Related pseudogenes have been found on chromosomes 3 and 14.
