An E.coli-derived zebrafish Cul1a/b recombinant protein (amino acids K436-A777) was used as the immunogen for the Zebrafish Cul1 antibody. This antibody will detect the a and b isoforms of Cul1.
Cullin 1 (Cul1) is a core component of the SCF (SKP1a€''Cullina€''F-box) E3 ubiquitin ligase complex, which plays a crucial role in regulating protein turnover via the ubiquitin-proteasome system. In zebrafish (Danio rerio), Cul1 is highly conserved and functionally analogous to its mammalian counterpart, mediating ubiquitination and subsequent proteasomal degradation of key regulatory proteins involved in cell cycle progression, signal transduction, and developmental processes.Cul1 functions as a scaffold protein, linking the SKP1 adaptor and the Rbx1 RING-finger protein, enabling the recruitment of specific F-box proteins that confer substrate specificity. Zebrafish cul1 expression is detected during early embryogenesis, particularly in proliferative tissues, reflecting its essential role in controlling cell division and differentiation during development.Functional studies in zebrafish have shown that loss of Cul1 activity can disrupt cell cycle control, leading to developmental defects and altered cell proliferation. Its evolutionary conservation and essential cellular functions make zebrafish Cul1 a valuable model for studying ubiquitin-mediated regulation in vertebrate development and disease models.
