CD8a Antibody, Clone: [SPM548], Mouse, Monoclonal

Artikelnummer: NSJ-V2386IHC-7ML
Artikelname: CD8a Antibody, Clone: [SPM548], Mouse, Monoclonal
Artikelnummer: NSJ-V2386IHC-7ML
Hersteller Artikelnummer: V2386IHC-7ML
Alternativnummer: NSJ-V2386IHC-7ML
Hersteller: NSJ Bioreagents
Wirt: Mouse
Kategorie: Antikörper
Applikation: IHC-P
Spezies Reaktivität: Human
Immunogen: Human CD8 recombinant protein was used as immunogen for this CD8a antibody.
CD8 molecule consists of two chains, termed alpha and beta chain, which are expressed as a disulphide-linked heterodimer or homodimer. CD8 is expressed on T cell subset (cytotoxic/suppressor T cells), thymocytes and NK cells.
Klonalität: Monoclonal
Klon-Bezeichnung: [SPM548]
UniProt: P01732
Reinheit: Protein G affinity chromatography
Formulierung: Prediluted in 1X PBS, 0.1 mg/ml BSA (US sourced), 0.05% sodium azide, *For IHC use only*
Antibody Type: Primary Antibody
Application Verdünnung: The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Anwendungsbeschreibung: Variations in protocols, secondaries and substrates may require the CD8a antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.