A cytoskeletal preparation from HeLa cells was used as the immunogen for the Cytokeratin 5/8 antibody.
It reacts with keratin 5 (58kDa) and keratin 8 (52.5kDa). Simple epithelia express cytokeratin 8 in combination with 18. On the other hand, basal cells of stratified epithelia express cytokeratin 5 paired with 14. This antibody therefore, reacts with a wide range of epithelia and their carcinomas.
Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
Anwendungsbeschreibung:
Optimal dilution of the Cytokeratin 5/8 antibody should be determined by the researcher.1. For staining of formalin-fixed tissues, digest sections with Trypsin at 1mg/ml PBS, 15 min at RT2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
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