CLUH Antibody, Unconjugated, Rabbit, Polyclonal

Produktübersicht

• Artikelname: CLUH Antibody, Unconjugated, Rabbit, Polyclonal
• Artikelnummer: PRS-13-454
• Hersteller Artikelnummer: 13-454
• Alternativnummer: PRS-13-454-100
• Hersteller: ProSci
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: IF, IHC, WB
• Spezies Reaktivität: Human, Mouse
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1120-1309 of human CLUH (NP_056044.3).
• Konjugation: Unconjugated
• Alternative Synonym: CLU1, clustered mitochondria protein homolog, clustered mitochondria (cluA/CLU1) homolog

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: batch dependent
• Molekulargewicht: Observed: 180kDa
• NCBI: 23277
• UniProt: O75153
• Puffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Formulierung: Liquid

Anwendungsbeschreibung

• Application Verdünnung: Optimal dilutions for each application to be determined by the researcher.
• Anwendungsbeschreibung: WB: 1:1000 - 1:4000IHC: 1:50 - 1:200IF: 1:50 - 1:200

Bilder

Western blot analysis of extracts of various cell lines, using CLUH antibody (13-454) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 15s.

Immunohistochemistry of paraffin-embedded human placenta using CLUH antibody (13-454) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human stomach using CLUH antibody (13-454) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse heart using CLUH antibody (13-454) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of NIH-3T3 cells using CLUH Polyclonal Antibody (13-454) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Immunofluorescence analysis of U-2 OS cells using CLUH Polyclonal Antibody (13-454) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.