MTHFD2 Antibody, Unconjugated, Rabbit, Polyclonal

Produktübersicht

• Artikelname: MTHFD2 Antibody, Unconjugated, Rabbit, Polyclonal
• Artikelnummer: PRS-13-620
• Hersteller Artikelnummer: 13-620
• Alternativnummer: PRS-13-620-100
• Hersteller: ProSci
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: IF, IHC, WB
• Spezies Reaktivität: Human, Mouse, Rat
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 40-250 of human MTHFD2 (NP_006627.2).
• Konjugation: Unconjugated
• Alternative Synonym: MTHFD2, NMDMC

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: batch dependent
• Molekulargewicht: Observed: 38kDa
• NCBI: 10797
• UniProt: P13995
• Puffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Formulierung: Liquid

Anwendungsbeschreibung

• Application Verdünnung: Optimal dilutions for each application to be determined by the researcher.
• Anwendungsbeschreibung: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200

Bilder

Western blot analysis of extracts of various cell lines, using MTHFD2 antibody (13-620) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s.

Immunohistochemistry of paraffin-embedded human mammary cancer using MTHFD2 antibody (13-620) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human liver cancer using MTHFD2 antibody (13-620) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded rat kidney using MTHFD2 antibody (13-620) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse kidney using MTHFD2 antibody (13-620) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of C6 cells using MTHFD2 antibody (13-620) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of NIH-3T3 cells using MTHFD2 antibody (13-620) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of U-2 OS cells using MTHFD2 antibody (13-620) at dilution of 1:100. Blue: DAPI for nuclear staining.