CTGF Antibody, Unconjugated, Rabbit, Polyclonal

Artikelnummer: PRS-13-902
Artikelname: CTGF Antibody, Unconjugated, Rabbit, Polyclonal
Artikelnummer: PRS-13-902
Hersteller Artikelnummer: 13-902
Alternativnummer: PRS-13-902-100
Hersteller: ProSci
Wirt: Rabbit
Kategorie: Antikörper
Applikation: IF, IHC, WB
Spezies Reaktivität: Human, Mouse, Rat
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 27-349 of human CTGF (NP_001892.1).
Konjugation: Unconjugated
Alternative Synonym: CCN2, NOV2, HCS24, IGFBP8, CCN2, Connective tissue growth factor, CCN family member 2, IBP-8
Klonalität: Polyclonal
Konzentration: batch dependent
Molekulargewicht: Observed: 38kDa
NCBI: 1490
UniProt: P29279
Puffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Formulierung: Liquid
Application Verdünnung: Optimal dilutions for each application to be determined by the researcher.
Anwendungsbeschreibung: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:20 - 1:50
Western blot analysis of extracts of various cell lines, using CTGF antibody (13-902) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 30s.
Immunohistochemistry of paraffin-embedded rat heart using CTGF antibody (13-902) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human breast using CTGF antibody (13-902) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse kidney using CTGF antibody (13-902) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of C6 cells using CTGF antibody (13-902) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH/3T3 cells using CTGF antibody (13-902) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U2OS cells using CTGF antibody (13-902) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U2OS cells using CTGF antibody (13-902) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of HeLa cells using CTGF antibody (13-902) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.