EZH2 Antibody, Unconjugated, Rabbit, Polyclonal

Artikelnummer: PRS-13-908
Artikelname: EZH2 Antibody, Unconjugated, Rabbit, Polyclonal
Artikelnummer: PRS-13-908
Hersteller Artikelnummer: 13-908
Alternativnummer: PRS-13-908-100
Hersteller: ProSci
Wirt: Rabbit
Kategorie: Antikörper
Applikation: IF, IHC, WB
Spezies Reaktivität: Human, Mouse, Rat
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human EZH2 (NP_001190176.1).
Konjugation: Unconjugated
Alternative Synonym: EZH2, WVS, ENX1, EZH1, KMT6, WVS2, ENX-1, EZH2b, KMT6A
Klonalität: Polyclonal
Konzentration: batch dependent
Molekulargewicht: Observed: 78-110kDa
NCBI: 2146
UniProt: Q15910
Puffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Formulierung: Liquid
Application Verdünnung: Optimal dilutions for each application to be determined by the researcher.
Anwendungsbeschreibung: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200
Western blot analysis of extracts of various cell lines, using EZH2 antibody (13-908) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.
Immunohistochemistry of paraffin-embedded rat testis using EZH2 antibody (13-908) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using EZH2 antibody (13-908) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human breast cancer using EZH2 antibody (13-908) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human gastric cancer using EZH2 antibody (13-908) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse testis using EZH2 antibody (13-908) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using EZH2 antibody (13-908) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using EZH2 antibody (13-908).