SQSTM1 / p62 Antibody, Unconjugated, Rabbit, Polyclonal

Produktübersicht

• Artikelname: SQSTM1 / p62 Antibody, Unconjugated, Rabbit, Polyclonal
• Artikelnummer: PRS-13-962
• Hersteller Artikelnummer: 13-962
• Alternativnummer: PRS-13-962-100
• Hersteller: ProSci
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: IF, IHC, IP, WB
• Spezies Reaktivität: Human, Mouse, Rat
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-440 of human SQSTM1 / p62 (NP_001135770.1).
• Konjugation: Unconjugated
• Alternative Synonym: SQSTM1 Antibody: p60, p62, A170, OSIL, PDB3, ZIP3, p62B, ORCA, Sequestosome-1, EBI3-associated protein of 60 kDa, EBIAP

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: batch dependent
• Molekulargewicht: Observed: 62kDa
• NCBI: 8878
• UniProt: Q13501
• Puffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Formulierung: Liquid

Anwendungsbeschreibung

• Application Verdünnung: Optimal dilutions for each application to be determined by the researcher.
• Anwendungsbeschreibung: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200IP: 1:50 - 1:200

Bilder

Western blot analysis of extracts of various cell lines, using SQSTM1 antibody (13-962) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.

Immunohistochemistry of paraffin-embedded human tonsil using SQSTM1 / p62 antibody (13-962) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human colon carcinoma using SQSTM1 / p62 antibody (13-962) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human tonsil using SQSTM1 / p62 antibody (13-962) at dilution of 1:150 (40x lens).

Immunohistochemistry of paraffin-embedded human colon carcinoma using SQSTM1 / p62 antibody (13-962) at dilution of 1:150 (40x lens).

Immunofluorescence analysis of HeLa cells using SQSTM1 / p62 antibody (13-962) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of NIH/3T3 cells using SQSTM1 / p62 antibody (13-962) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunoprecipitation analysis of 200ug extracts of C6 cells using 3ug SQSTM1 / p62 antibody (13-962). Western blot was performed from the immunoprecipitate using SQSTM1 / p62 antibody (13-962) at a dilition of 1:1000.