EGFR Antibody, KO Validated, Unconjugated, Rabbit, Polyclonal

Produktübersicht

• Artikelname: EGFR Antibody, KO Validated, Unconjugated, Rabbit, Polyclonal
• Artikelnummer: PRS-13-994
• Hersteller Artikelnummer: 13-994
• Alternativnummer: PRS-13-994-100
• Hersteller: ProSci
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: IF, IHC, IP, WB
• Spezies Reaktivität: Human, Mouse, Rat
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1021-1210 of human EGFR (NP_005219.2).
• Konjugation: Unconjugated
• Alternative Synonym: Epidermal growth factor receptor, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1, EGFR, ERBB, ERBB1, HER1

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: batch dependent
• Molekulargewicht: Observed: 175kDa
• NCBI: 1956
• UniProt: P00533
• Puffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Formulierung: Liquid

Anwendungsbeschreibung

• Application Verdünnung: Optimal dilutions for each application to be determined by the researcher.
• Anwendungsbeschreibung: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200IP: 1:50 - 1:100

Bilder

Western blot analysis of extracts of OVCAR3 cells, using EGFR antibody (13-994) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 3s.

Western blot analysis of extracts from normal (control) and EGFR knockout (KO) HeLa cells, using EGFR antibody (13-994) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 3s.

Immunohistochemistry of paraffin-embedded human prostate using EGFR antibody (13-994) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse brain using EGFR antibody (13-994) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of HeLa cells using EGFR antibody (13-994) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of NIH/3T3 cells using EGFR antibody (13-994) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of PC12 cells using EGFR antibody (13-994) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunoprecipitation analysis of 200ug extracts of HeLa cells using 3ug EGFR antibody (13-994). Western blot was performed from the immunoprecipitate using EGFR antibody (13-994) at a dilition of 1:1000.