HNRNPA2B1 Antibody, KO Validated, Unconjugated, Rabbit, Polyclonal

Artikelnummer: PRS-14-069
Artikelname: HNRNPA2B1 Antibody, KO Validated, Unconjugated, Rabbit, Polyclonal
Artikelnummer: PRS-14-069
Hersteller Artikelnummer: 14-069
Alternativnummer: PRS-14-069-100
Hersteller: ProSci
Wirt: Rabbit
Kategorie: Antikörper
Applikation: IF, IHC, WB
Spezies Reaktivität: Human, Mouse, Rat
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-180 of human HNRNPA2B1 (NP_002128.1).
Konjugation: Unconjugated
Alternative Synonym: HNRNPA2B1, DKFZp779B0244, FLJ22720, HNRNPA2, HNRNPB1, HNRPA2, HNRPA2B1, HNRPB1, RNPA2, SNRPB1, IBMPFD2
Klonalität: Polyclonal
Konzentration: batch dependent
Molekulargewicht: Observed: 37kDa
NCBI: 3181
UniProt: P22626
Puffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Formulierung: Liquid
Application Verdünnung: Optimal dilutions for each application to be determined by the researcher.
Anwendungsbeschreibung: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200
Western blot analysis of extracts of various cell lines, using HNRNPA2B1 antibody (14-069) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Western blot analysis of extracts from normal (control) and HNRNPA2B1 knockout (KO) 293T cells, using HNRNPA2B1 antibody (14-069) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s.
Immunohistochemistry of paraffin-embedded mouse brain using HNRNPA2B1 Antibody (14-069) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse kidney using HNRNPA2B1 Antibody (14-069) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using HNRNPA2B1 Antibody (14-069) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat kidney using HNRNPA2B1 Antibody (14-069) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat liver using HNRNPA2B1 Antibody (14-069) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human colon using HNRNPA2B1 Antibody (14-069) at dilution of 1:200 (40x lens).
Immunofluorescence analysis of HeLa cells using HNRNPA2B1 antibody (14-069). Blue: DAPI for nuclear staining.