HAL Antibody, Unconjugated, Rabbit, Polyclonal

Produktübersicht

• Artikelname: HAL Antibody, Unconjugated, Rabbit, Polyclonal
• Artikelnummer: PRS-14-749
• Hersteller Artikelnummer: 14-749
• Alternativnummer: PRS-14-749-100
• Hersteller: ProSci
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: IF, IHC, WB
• Spezies Reaktivität: Human, Mouse, Rat
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human HAL (NP_002099.1).
• Konjugation: Unconjugated
• Alternative Synonym: HAL, HIS, HSTD, histidase

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: batch dependent
• Molekulargewicht: Observed: 73kDa
• NCBI: 3034
• UniProt: P42357
• Puffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Formulierung: Liquid

Anwendungsbeschreibung

• Application Verdünnung: Optimal dilutions for each application to be determined by the researcher.
• Anwendungsbeschreibung: WB: 1:500 - 1:2000IHC: 1:50 - 1:100IF: 1:50 - 1:100

Bilder

Western blot analysis of extracts of various cell lines, using HAL antibody (14-749) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 90s.

Immunohistochemistry of paraffin-embedded rat kidney using HAL antibody (14-749) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human lung cancer using HAL antibody (14-749) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human thyroid cancer using HAL antibody (14-749) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse heart using HAL antibody (14-749) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of C6 cells using HAL Polyclonal Antibody (14-749) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.