DDX5 Antibody, Unconjugated, Rabbit, Polyclonal

Artikelnummer: PRS-14-895
Artikelname: DDX5 Antibody, Unconjugated, Rabbit, Polyclonal
Artikelnummer: PRS-14-895
Hersteller Artikelnummer: 14-895
Alternativnummer: PRS-14-895-100
Hersteller: ProSci
Wirt: Rabbit
Kategorie: Antikörper
Applikation: IF, IHC, WB
Spezies Reaktivität: Human, Mouse, Rat
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-614 of human DDX5 (NP_004387.1).
Konjugation: Unconjugated
Alternative Synonym: DDX5, p68, HLR1, G17P1, HUMP68
Klonalität: Polyclonal
Konzentration: batch dependent
Molekulargewicht: Observed: 69kDa
NCBI: 1655
UniProt: P17844
Puffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Formulierung: Liquid
Application Verdünnung: Optimal dilutions for each application to be determined by the researcher.
Anwendungsbeschreibung: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200
Western blot analysis of extracts of various cell lines, using DDX5 antibody (14-895) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 5s.
Immunohistochemistry of paraffin-embedded rat lung using DDX5 antibody (14-895) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human lung cancer using DDX5 antibody (14-895) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human oophoroma using DDX5 antibody (14-895) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human esophagus using DDX5 antibody (14-895) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse lung using DDX5 antibody (14-895) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse liver using DDX5 antibody (14-895) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of HeLa cells using DDX5 antibody (14-895). Blue: DAPI for nuclear staining.