MCM5 Antibody, Unconjugated, Rabbit, Polyclonal

Produktübersicht

• Artikelname: MCM5 Antibody, Unconjugated, Rabbit, Polyclonal
• Artikelnummer: PRS-15-036
• Hersteller Artikelnummer: 15-036
• Alternativnummer: PRS-15-036-100
• Hersteller: ProSci
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: IF, IHC, WB
• Spezies Reaktivität: Human, Mouse, Rat
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-230 of human MCM5 (NP_006730.2).
• Konjugation: Unconjugated
• Alternative Synonym: MCM5, CDC46, P1-CDC46

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: batch dependent
• Molekulargewicht: Observed: 100kDa
• NCBI: 4174
• UniProt: P33992
• Puffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Formulierung: Liquid

Anwendungsbeschreibung

• Application Verdünnung: Optimal dilutions for each application to be determined by the researcher.
• Anwendungsbeschreibung: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200

Bilder

Western blot analysis of extracts of various cell lines, using MCM5 antibody (15-036) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s.

Immunohistochemistry of paraffin-embedded human lung cancer using MCM5 antibody (15-036) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human appendix using MCM5 antibody (15-036) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of C6 cells using MCM5 antibody (15-036) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of NIH-3T3 cells using MCM5 antibody (15-036) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of U-2 OS cells using MCM5 antibody (15-036) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of C6 cells using MCM5 antibody (15-036) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of NIH-3T3 cells using MCM5 antibody (15-036) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of U-2 OS cells using MCM5 antibody (15-036) at dilution of 1:100. Blue: DAPI for nuclear staining.