PRKCB Antibody, Unconjugated, Rabbit, Polyclonal

Produktübersicht

• Artikelname: PRKCB Antibody, Unconjugated, Rabbit, Polyclonal
• Artikelnummer: PRS-15-109
• Hersteller Artikelnummer: 15-109
• Alternativnummer: PRS-15-109-100
• Hersteller: ProSci
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: IF, IHC, WB
• Spezies Reaktivität: Human, Mouse, Rat
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human PRKCB (NP_002729.2).
• Konjugation: Unconjugated
• Alternative Synonym: PRKCB, protein kinase C, beta, MGC41878, PKC-beta, PKCB, PRKCB1, PRKCB2, OTTHUMP00000162377, OTTHUMP00000162378, PKC-B, protein kinase C beta type, protein kinase C, beta 1 polypeptide

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: batch dependent
• Molekulargewicht: Observed: 77kDa
• NCBI: 5579
• UniProt: P05771
• Puffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Formulierung: Liquid

Anwendungsbeschreibung

• Application Verdünnung: Optimal dilutions for each application to be determined by the researcher.
• Anwendungsbeschreibung: WB: 1:500 - 1:2000IHC: 1:50 - 1:100IF: 1:50 - 1:100

Bilder

Western blot analysis of extracts of mouse liver, using PRKCB antibody (15-109) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 50s.

Immunohistochemistry of paraffin-embedded mouse lung using PRKCB antibody (15-109) at dilution of 1:200 (40x lens).

Immunohistochemistry of paraffin-embedded mouse kidney using PRKCB antibody (15-109) at dilution of 1:200 (40x lens).

Immunofluorescence analysis of C6 cells using PRKCB Polyclonal Antibody (15-109) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Immunofluorescence analysis of HeLa cells using PRKCB Polyclonal Antibody (15-109) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Immunofluorescence analysis of NIH-3T3 cells using PRKCB Polyclonal Antibody (15-109) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.