HSF1 Antibody, Unconjugated, Rabbit, Polyclonal

Produktübersicht

• Artikelname: HSF1 Antibody, Unconjugated, Rabbit, Polyclonal
• Artikelnummer: PRS-15-191
• Hersteller Artikelnummer: 15-191
• Alternativnummer: PRS-15-191-100
• Hersteller: ProSci
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: IF, IHC, WB
• Spezies Reaktivität: Human, Mouse, Rat
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 350-529 of human HSF1 (NP_005517.1).
• Konjugation: Unconjugated
• Alternative Synonym: HSF1, HSTF1

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: batch dependent
• Molekulargewicht: Observed: 90kDa
• NCBI: 3297
• UniProt: Q00613
• Puffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Formulierung: Liquid

Anwendungsbeschreibung

• Application Verdünnung: Optimal dilutions for each application to be determined by the researcher.
• Anwendungsbeschreibung: WB: 1:500 - 1:2000IHC: 1:50 - 1:100IF: 1:50 - 1:100

Bilder

Western blot analysis of extracts of various cell lines, using HSF1 antibody (15-191) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.

Immunohistochemistry of paraffin-embedded rat liver using HSF1 antibody (15-191) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded rat brain using HSF1 antibody (15-191) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded rat spleen using HSF1 antibody (15-191) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human placenta using HSF1 antibody (15-191) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse kidney using HSF1 antibody (15-191) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of C6 cells using HSF1 antibody (15-191) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of L929 cells using HSF1 antibody (15-191) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of U2OS cells using HSF1 antibody (15-191) at dilution of 1:100. Blue: DAPI for nuclear staining.