GANAB Antibody, Unconjugated, Rabbit, Polyclonal

Artikelnummer: PRS-15-233
Artikelname: GANAB Antibody, Unconjugated, Rabbit, Polyclonal
Artikelnummer: PRS-15-233
Hersteller Artikelnummer: 15-233
Alternativnummer: PRS-15-233-100
Hersteller: ProSci
Wirt: Rabbit
Kategorie: Antikörper
Applikation: IF, IHC, WB
Spezies Reaktivität: Human, Mouse, Rat
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human GANAB (NP_001265123.1).
Konjugation: Unconjugated
Alternative Synonym: GANAB, G2AN, Glucosidase, alpha, Glucosidase, alpha neutral AB, Neutral alpha-glucosidase AB, Alpha-glucosidase 2, Glucosidase II subunit alpha, GLUII, KIAA0088, Neutral AB
Klonalität: Polyclonal
Konzentration: batch dependent
Molekulargewicht: Observed: 112kDa
NCBI: 23193
UniProt: Q14697
Puffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Formulierung: Liquid
Application Verdünnung: Optimal dilutions for each application to be determined by the researcher.
Anwendungsbeschreibung: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200
Western blot analysis of extracts of various cell lines, using GANAB antibody (15-233) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.
Immunohistochemistry of paraffin-embedded rat brain using GANAB antibody (15-233) at dilution of 1:150 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using GANAB antibody (15-233) at dilution of 1:150 (40x lens).
Immunofluorescence analysis of C6 cells using GANAB antibody (15-233) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH-3T3 cells using GANAB antibody (15-233) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U-2 OS cells using GANAB antibody (15-233) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of C6 cells using GANAB antibody (15-233) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH-3T3 cells using GANAB antibody (15-233) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U-2 OS cells using GANAB antibody (15-233) at dilution of 1:100. Blue: DAPI for nuclear staining.