COX15 Antibody, Unconjugated, Rabbit, Polyclonal

Artikelnummer: PRS-15-661
Artikelname: COX15 Antibody, Unconjugated, Rabbit, Polyclonal
Artikelnummer: PRS-15-661
Hersteller Artikelnummer: 15-661
Alternativnummer: PRS-15-661-100
Hersteller: ProSci
Wirt: Rabbit
Kategorie: Antikörper
Applikation: IF, IHC, WB
Spezies Reaktivität: Human, Mouse, Rat
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 150-250 of human COX15 (NP_510870.1).
Konjugation: Unconjugated
Alternative Synonym: COX15, CEMCOX2
Klonalität: Polyclonal
Konzentration: batch dependent
Molekulargewicht: Observed: 60kDa
NCBI: 1355
UniProt: Q7KZN9
Puffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Formulierung: Liquid
Application Verdünnung: Optimal dilutions for each application to be determined by the researcher.
Anwendungsbeschreibung: WB: 1:500 - 1:2000IHC: 1:50 - 1:100IF: 1:50 - 1:100
Western blot analysis of extracts of various cell lines, using COX15 antibody (15-661) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 5s.
Immunohistochemistry of paraffin-embedded rat spleen using COX15 antibody (15-661) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat heart using COX15 antibody (15-661) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human lung cancer using COX15 antibody (15-661) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human thyroid cancer using COX15 antibody (15-661) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human placenta using COX15 antibody (15-661) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse spleen using COX15 antibody (15-661) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse heart using COX15 antibody (15-661) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of L929 cells using COX15 Polyclonal Antibody (15-661) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.