BAG3 Antibody, Unconjugated, Rabbit, Polyclonal

Artikelnummer: PRS-15-750
Artikelname: BAG3 Antibody, Unconjugated, Rabbit, Polyclonal
Artikelnummer: PRS-15-750
Hersteller Artikelnummer: 15-750
Alternativnummer: PRS-15-750-100
Hersteller: ProSci
Wirt: Rabbit
Kategorie: Antikörper
Applikation: IF, IHC, IP, WB
Spezies Reaktivität: Human, Mouse, Rat
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 380-575 of human BAG3 (NP_004272.2).
Konjugation: Unconjugated
Alternative Synonym: BAG3, BCL2-associated athanogene 3 , BAG-3, BIS, CAIR-1, MGC104307, BAG-family molecular chaperone regulator-3, BCL2-binding athanogene 3, Bcl-2-binding protein, docking protein CAIR-1
Klonalität: Polyclonal
Konzentration: batch dependent
Molekulargewicht: Observed: 80kDa
NCBI: 9531
UniProt: O95817
Puffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Formulierung: Liquid
Application Verdünnung: Optimal dilutions for each application to be determined by the researcher.
Anwendungsbeschreibung: WB: 1:500 - 1:2000IHC: 1:50 - 1:100IF: 1:50 - 1:100IP: 1:50 - 1:100
Western blot analysis of extracts of various cell lines, using BAG3 antibody (15-750) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.
Immunohistochemistry of paraffin-embedded rat heart using BAG3 antibody (15-750) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human lung using BAG3 antibody (15-750) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human lung cancer using BAG3 antibody (15-750) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human breast cancer using BAG3 antibody (15-750) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse heart using BAG3 antibody (15-750) at dilution of 1:100 (40x lens).