VDAC1 / Porin Antibody, Unconjugated, Rabbit, Polyclonal

Artikelnummer: PRS-16-208
Artikelname: VDAC1 / Porin Antibody, Unconjugated, Rabbit, Polyclonal
Artikelnummer: PRS-16-208
Hersteller Artikelnummer: 16-208
Alternativnummer: PRS-16-208-100
Hersteller: ProSci
Wirt: Rabbit
Kategorie: Antikörper
Applikation: IF, IHC, WB
Spezies Reaktivität: Human, Mouse, Rat
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-283 of human VDAC1 / Porin (NP_003365.1).
Konjugation: Unconjugated
Alternative Synonym: VDAC1, PORIN, VDAC-1
Klonalität: Polyclonal
Konzentration: batch dependent
Molekulargewicht: Observed: 36kDa
NCBI: 7416
UniProt: P21796
Puffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Formulierung: Liquid
Application Verdünnung: Optimal dilutions for each application to be determined by the researcher.
Anwendungsbeschreibung: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200
Western blot analysis of extracts of various cell lines, using VDAC1 / Porin antibody (16-208) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.
Immunohistochemistry of paraffin-embedded rat heart using VDAC1 / Porin antibody (16-208) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human colon carcinoma using VDAC1 / Porin antibody (16-208) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using VDAC1 / Porin antibody (16-208) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of NIH/3T3 cells using VDAC1 / Porin antibody (16-208) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of PC12 cells using VDAC1 / Porin antibody (16-208) at dilution of 1:100. Blue: DAPI for nuclear staining.