TriMethyl-Histone H3-K27 mAb, Unconjugated, Mouse, Monoclonal

Produktübersicht

• Artikelname: TriMethyl-Histone H3-K27 mAb, Unconjugated, Mouse, Monoclonal
• Artikelnummer: PRS-16-486
• Hersteller Artikelnummer: 16-486
• Alternativnummer: PRS-16-486-100
• Hersteller: ProSci
• Wirt: Mouse
• Kategorie: Antikörper
• Applikation: ChIP, IF, IHC, IP, WB
• Spezies Reaktivität: Human, Mouse, Rat
• Immunogen: A synthetic methylated peptide corresponding to residues surrounding K27 of human histone H3
• Konjugation: Unconjugated
• Alternative Synonym: H3F3A, H3t, H3.4, H3/g, H3FT

Produkteigenschaften

• Klonalität: Monoclonal
• Konzentration: batch dependent
• NCBI: 8290
• UniProt: Q16695
• Puffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Formulierung: Liquid

Anwendungsbeschreibung

• Application Verdünnung: Optimal dilutions for each application to be determined by the researcher.
• Anwendungsbeschreibung: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200IP: 1:50 - 1:200ChIP: 1:20 - 1:100

Bilder

Western blot analysis of extracts of various cell lines, using TriMethyl-Histone H3-K27 antibody (16-486) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 5s.

Dot-blot analysis of all sorts of methylation peptides using TriMethyl-Histone H3-K27 antibody (16-486) at 1:1000 dilution.

Immunohistochemistry of paraffin-embedded human colon carcinoma using TriMethyl-Histone H3-K27 antibody (16-486) at dilution of 1:25 (40x lens).

Immunohistochemistry of paraffin-embedded mouse brain using TriMethyl-Histone H3-K27 antibody (16-486) at dilution of 1:25 (40x lens).

Immunohistochemistry of paraffin-embedded rat brain using TriMethyl-Histone H3-K27 antibody (16-486) at dilution of 1:25 (40x lens).

Immunofluorescence analysis of C6 cells using TriMethyl-Histone H3-K27 antibody (16-486) at dilution of 1:25. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of NIH/3T3 cells using TriMethyl-Histone H3-K27 antibody (16-486) at dilution of 1:25. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of U-2OS cells using TriMethyl-Histone H3-K27 antibody (16-486) at dilution of 1:25. Blue: DAPI for nuclear staining.