IL2 Antibody, Unconjugated, Rabbit, Polyclonal

Produktübersicht

• Artikelname: IL2 Antibody, Unconjugated, Rabbit, Polyclonal
• Artikelnummer: PRS-16-547
• Hersteller Artikelnummer: 16-547
• Alternativnummer: PRS-16-547-100
• Hersteller: ProSci
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: IHC, WB
• Spezies Reaktivität: Human, Mouse, Rat
• Immunogen: Recombinant protein of human IL2
• Konjugation: Unconjugated
• Alternative Synonym: Interleukin 2Interleukin-2, T-cell growth factor, IL-2, TCGF, lymphokine

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: batch dependent
• Molekulargewicht: Observed: 18kDa
• NCBI: 3558
• UniProt: P60568
• Puffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Formulierung: Liquid

Anwendungsbeschreibung

• Application Verdünnung: Optimal dilutions for each application to be determined by the researcher.
• Anwendungsbeschreibung: WB: 1:500 - 1:2000IHC: 1:50 - 1:200

Bilder

Immunohistochemistry of paraffin-embedded mouse lung using IL2 antibody (16-547) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse spleen using IL2 antibody (16-547) at dilution of 1:100 (40x lens).

Western blot analysis of extracts of various cell lines, using IL2 antibody (16-547) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.

Western blot analysis of extracts of Raw264.7 cells, using IL2 antibody (16-547) at 1:1000 dilution. Raw264.7 cells were treated by LPS for 6 hours and Brefeldin A for 3 hours of stimulation.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.

Immunohistochemistry of paraffin-embedded human tonsil using IL2 antibody (16-547) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human lung using IL2 antibody (16-547) at dilution of 1:100 (40x lens).