Optimal dilutions for each application to be determined by the researcher.
Anwendungsbeschreibung:
WB: 1:500 - 1:2000IHC: 1:50 - 1:200
Western blot analysis of extracts of HepG2 cells, using NUBP1 antibody (16-590) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Immunohistochemistry of paraffin-embedded rat heart using NUBP1 antibody (16-590) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human thyroid cancer using NUBP1 antibody (16-590) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human colon carcinoma using NUBP1 antibody (16-590) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse liver using NUBP1 antibody (16-590) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse kidney using NUBP1 antibody (16-590) at dilution of 1:100 (40x lens).
* Mehrwertsteuer und Versandkosten nicht enthalten. Irrtümer und Preisänderungen vorbehalten