Lamin B1 Antibody, Unconjugated, Rabbit, Polyclonal

Artikelnummer: PRS-16-793
Artikelname: Lamin B1 Antibody, Unconjugated, Rabbit, Polyclonal
Artikelnummer: PRS-16-793
Hersteller Artikelnummer: 16-793
Alternativnummer: PRS-16-793-100
Hersteller: ProSci
Wirt: Rabbit
Kategorie: Antikörper
Applikation: IF, IHC, WB
Spezies Reaktivität: Human, Mouse, Rat
Immunogen: A synthetic peptide of human Lamin B1
Konjugation: Unconjugated
Alternative Synonym: LMNB1, lamin B1, ADLD, LMN, LMN2, LMNB, MGC111419, OTTHUMP00000159218, lamin-B1
Klonalität: Polyclonal
Konzentration: batch dependent
Molekulargewicht: Observed: 75kDa
NCBI: 4001
UniProt: P20700
Puffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Formulierung: Liquid
Application Verdünnung: Optimal dilutions for each application to be determined by the researcher.
Anwendungsbeschreibung: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200
Western blot analysis of extracts of various cell lines, using Lamin B1 antibody (16-793) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s.
Immunohistochemistry of paraffin-embedded human breast using Lamin B1 antibody (16-793) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human tonsil using Lamin B1 antibody (16-793) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using Lamin B1 antibody (16-793) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using Lamin B1 antibody (16-793) at dilution of 1:200 (40x lens).
Immunofluorescence analysis of U2OS cells using Lamin B1 antibody (16-793) at dilution of 1:100. Blue: DAPI for nuclear staining.