PDHA1 Antibody, Unconjugated, Rabbit, Polyclonal

Produktübersicht

• Artikelname: PDHA1 Antibody, Unconjugated, Rabbit, Polyclonal
• Artikelnummer: PRS-18-369
• Hersteller Artikelnummer: 18-369
• Alternativnummer: PRS-18-369-100
• Hersteller: ProSci
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: IF, IHC, IP, WB
• Spezies Reaktivität: Human, Mouse, Rat
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 30-390 of human PDHA1 (NP_000275.1).
• Konjugation: Unconjugated
• Alternative Synonym: PDHA1, PDHA, PDHCE1A, PHE1A

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: batch dependent
• Molekulargewicht: Observed: 45kDa
• NCBI: 5160
• UniProt: P08559
• Puffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Formulierung: Liquid

Anwendungsbeschreibung

• Application Verdünnung: Optimal dilutions for each application to be determined by the researcher.
• Anwendungsbeschreibung: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200IP: 1:20 - 1:50

Bilder

Western blot analysis of extracts of various cell lines, using PDHA1 antibody (18-369) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

Immunohistochemistry of paraffin-embedded human colon carcinoma using PDHA1 antibody (18-369) at dilution of 1:200 (40x lens).

Immunohistochemistry of paraffin-embedded human mammary cancer using PDHA1 antibody (18-369) at dilution of 1:200 (40x lens).

Immunohistochemistry of paraffin-embedded human stomach using PDHA1 antibody (18-369) at dilution of 1:200 (40x lens).

Immunofluorescence analysis of NIH-3T3 cells using PDHA1 antibody (18-369) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of U-2 OS cells using PDHA1 antibody (18-369) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunoprecipitation analysis of 200ug extracts of U-87MG cells using 3ug PDHA1 antibody (18-369). Western blot was performed from the immunoprecipitate using PDHA1 antibody (18-369) at a dilition of 1:1000.