BMPR1B Antibody, Unconjugated, Rabbit, Polyclonal

Artikelnummer: PRS-18-442
Artikelname: BMPR1B Antibody, Unconjugated, Rabbit, Polyclonal
Artikelnummer: PRS-18-442
Hersteller Artikelnummer: 18-442
Alternativnummer: PRS-18-442-100
Hersteller: ProSci
Wirt: Rabbit
Kategorie: Antikörper
Applikation: IF, IHC, WB
Spezies Reaktivität: Human, Mouse, Rat
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-126 of human BMPR1B (NP_001194.1).
Konjugation: Unconjugated
Alternative Synonym: Bone morphogenetic protein receptor type-1B, BMP type-1B receptor, BMPR-1B, CDw293, BMPR1B
Klonalität: Polyclonal
Konzentration: batch dependent
Molekulargewicht: Observed: 55kDa
NCBI: 658
UniProt: O00238
Puffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Formulierung: Liquid
Application Verdünnung: Optimal dilutions for each application to be determined by the researcher.
Anwendungsbeschreibung: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200
Western blot analysis of extracts of various cell lines, using BMPR1B Antibody (18-442) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Immunohistochemistry of paraffin-embedded human tonsil using BMPR1B antibody (18-442) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human gastric cancer using BMPR1B antibody (18-442) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded rat ovary using BMPR1B antibody (18-442) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded mouse liver using BMPR1B antibody (18-442) at dilution of 1:200 (40x lens).
Immunofluorescence analysis of A431 cells using BMPR1B antibody (18-442) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.