Histone H3 Antibody, Unconjugated, Rabbit, Polyclonal

Produktübersicht

• Artikelname: Histone H3 Antibody, Unconjugated, Rabbit, Polyclonal
• Artikelnummer: PRS-18-615
• Hersteller Artikelnummer: 18-615
• Alternativnummer: PRS-18-615-100
• Hersteller: ProSci
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ChIP, IF, IHC, WB
• Spezies Reaktivität: Human, Mouse, Other, Rat
• Immunogen: A synthetic peptide corresponding to a sequence within amino acids 50 to the C-terminus of human HIST3H3 (NP_003484.1).
• Konjugation: Unconjugated
• Alternative Synonym: H3F3A, H3t, H3.4, H3/g, H3FT

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: batch dependent
• Molekulargewicht: Observed: 17kDa
• NCBI: 8290
• UniProt: Q16695
• Puffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Formulierung: Liquid

Anwendungsbeschreibung

• Application Verdünnung: Optimal dilutions for each application to be determined by the researcher.
• Anwendungsbeschreibung: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200ChIP: 1:50 - 1:200

Bilder

Western blot analysis of extracts of various cell lines, using HIST3H3 antibody (18-615) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.

Immunohistochemistry of paraffin-embedded human placenta using Histone H3 antibody (18-615) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse kidney using Histone H3 antibody (18-615) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded rat brain using Histone H3 antibody (18-615) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of U2OS cells using HIST3H3 antibody (18-615) at dilution of 1:100. Blue: DAPI for nuclear staining.

Chromatin immunoprecipitation analysis of extracts of HeLa cell line, using HIST3H3 antibody (18-615) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.