mTOR Antibody, Unconjugated, Rabbit, Polyclonal

Produktübersicht

• Artikelname: mTOR Antibody, Unconjugated, Rabbit, Polyclonal
• Artikelnummer: PRS-18-651
• Hersteller Artikelnummer: 18-651
• Alternativnummer: PRS-18-651-100
• Hersteller: ProSci
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: IF, IP, WB
• Spezies Reaktivität: Human, Mouse, Rat
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human mTOR (NP_004949.1).
• Konjugation: Unconjugated
• Alternative Synonym: TOR Antibody: FRAP, FRAP1, FRAP2, RAFT1, RAPT1, FRAP, FK506-binding protein 12-rapamycin complex-associated protein 1, mTOR

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: batch dependent
• Molekulargewicht: Observed: 289kDa
• NCBI: 2475
• UniProt: P42345
• Puffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Formulierung: Liquid

Anwendungsbeschreibung

• Application Verdünnung: Optimal dilutions for each application to be determined by the researcher.
• Anwendungsbeschreibung: WB: 1:500 - 1:2000IF: 1:50 - 1:200IP: 1:50 - 1:100

Bilder

Western blot analysis of extracts of various cell lines, using mTOR antibody (18-651) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.

Immunofluorescence analysis of HeLa cells using mTOR antibody (18-651) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of PC12 cells using mTOR antibody (18-651) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of C6 cells using MTOR antibody (18-651) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of NIH/3T3 cells using MTOR antibody (18-651) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunoprecipitation analysis of 200ug extracts of HeLa cells, using 3 ug mTOR antibody (18-651). Western blot was performed from the immunoprecipitate using mTOR antibody (18-651) at a dilition of 1:1000.