AIF Antibody, KO Validated, Unconjugated, Rabbit, Polyclonal

Produktübersicht

• Artikelname: AIF Antibody, KO Validated, Unconjugated, Rabbit, Polyclonal
• Artikelnummer: PRS-18-715
• Hersteller Artikelnummer: 18-715
• Alternativnummer: PRS-18-715-100
• Hersteller: ProSci
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: IF, IHC, IP, WB
• Spezies Reaktivität: Human, Mouse, Rat
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 334-613 of human AIF (NP_004199.1).
• Konjugation: Unconjugated
• Alternative Synonym: AIF Antibody: CNK, KSR, CNK1, Connector enhancer of kinase suppressor of ras 1, CNK homolog protein 1, Connector enhancer of KSR 1

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: batch dependent
• Molekulargewicht: Observed: 67kDa
• NCBI: 9131
• UniProt: O95831
• Puffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Formulierung: Liquid

Anwendungsbeschreibung

• Application Verdünnung: Optimal dilutions for each application to be determined by the researcher.
• Anwendungsbeschreibung: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200IP: 1:50 - 1:100

Bilder

Western blot analysis of extracts of various cell lines, using AIF antibody (18-715) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

Western blot analysis of extracts from normal (control) and AIF knockout (KO) 293T cells, using AIF antibody (18-715) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s.

Immunohistochemistry of paraffin-embedded rat lung using AIF antibody (18-715) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human liver cancer using AIF antibody (18-715) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human stomach using AIF antibody (18-715) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse testis using AIF antibody (18-715) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of U2OS cells using AIF antibody (18-715). Blue: DAPI for nuclear staining.

Immunofluorescence analysis of HeLa cells using AIF antibody (18-715) at dilution of 1:200 (60x lens). Blue: DAPI for nuclear staining.

Confocal immunofluorescence analysis of Hela cells using AIF antibody (18-715) at dilution of 1:200. Blue: DAPI for nuclear staining.