Occludin Antibody, Unconjugated, Rabbit, Polyclonal

Produktübersicht

• Artikelname: Occludin Antibody, Unconjugated, Rabbit, Polyclonal
• Artikelnummer: PRS-18-733
• Hersteller Artikelnummer: 18-733
• Alternativnummer: PRS-18-733-100
• Hersteller: ProSci
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: WB
• Spezies Reaktivität: Human, Mouse, Rat
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 263-522 of human Occludin (NP_002529.1).
• Konjugation: Unconjugated
• Alternative Synonym: Occludin, OCLN

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: batch dependent
• Molekulargewicht: Observed: 59kDa
• NCBI: 100506658
• UniProt: Q16625
• Puffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Formulierung: Liquid

Anwendungsbeschreibung

• Application Verdünnung: Optimal dilutions for each application to be determined by the researcher.
• Anwendungsbeschreibung: WB: 1:1000 - 1:2000

Bilder

Western blot analysis of extracts of various cell lines, using Occludin antibody (18-733) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s.

Immunohistochemistry of paraffin-embedded rat pancreas using Occludin antibody (18-733) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse kidney using Occludin antibody (18-733) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of HeLa cells using Occludin antibody (18-733) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of NIH/3T3 cells using Occludin antibody (18-733) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of PC12 cells using Occludin antibody (18-733) at dilution of 1:100. Blue: DAPI for nuclear staining.