Symmetric DiMethyl-Histone H3-R26 pAb, Unconjugated, Rabbit, Polyclonal

Artikelnummer: PRS-18-965
Artikelname: Symmetric DiMethyl-Histone H3-R26 pAb, Unconjugated, Rabbit, Polyclonal
Artikelnummer: PRS-18-965
Hersteller Artikelnummer: 18-965
Alternativnummer: PRS-18-965-100
Hersteller: ProSci
Wirt: Rabbit
Kategorie: Antikörper
Applikation: IF, WB
Spezies Reaktivität: Mouse, Rat
Immunogen: A synthetic methylated peptide corresponding to residues surrounding Arg26 of human histone H3
Konjugation: Unconjugated
Alternative Synonym: H3/b, H3FB, ERBB, ERBB1, HER1, H3/a, H3/c, H3/d, H3/f, H3/h
Klonalität: Polyclonal
Konzentration: batch dependent
Molekulargewicht: Observed: 17kDa
NCBI: 8356
UniProt: P68431
Puffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Formulierung: Liquid
Application Verdünnung: Optimal dilutions for each application to be determined by the researcher.
Anwendungsbeschreibung: WB: 1:500 - 1:2000IF: 1:50 - 1:200
Western blot analysis of extracts of various cell lines, using Symmetric DiMethyl-Histone H3-R26 antibody (18-965).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Dot-blot analysis of all sorts of methylation peptides using Symmetric DiMethyl-Histone H3-R26 antibody (18-965).
Immunofluorescence analysis of 293T cells using Symmetric DiMethyl-Histone H3-R26 antibody (18-965). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of C6 cells using Symmetric DiMethyl-Histone H3-R26 antibody (18-965) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of HeLa cells using Symmetric DiMethyl-Histone H3-R26 antibody (18-965) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH/3T3 cells using Symmetric DiMethyl-Histone H3-R26 antibody (18-965) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of C6 cells using Symmetric DiMethyl-Histone H3-R26 antibody (18-965) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of HeLa cells using Symmetric DiMethyl-Histone H3-R26 antibody (18-965) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH/3T3 cells using Symmetric DiMethyl-Histone H3-R26 antibody (18-965) at dilution of 1:100. Blue: DAPI for nuclear staining.