ACAT1 Antibody, Unconjugated, Rabbit, Polyclonal

Produktübersicht

• Artikelname: ACAT1 Antibody, Unconjugated, Rabbit, Polyclonal
• Artikelnummer: PRS-19-597
• Hersteller Artikelnummer: 19-597
• Alternativnummer: PRS-19-597-100
• Hersteller: ProSci
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: IF, IHC, WB
• Spezies Reaktivität: Human, Mouse, Rat
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-145 of human ACAT1 (NP_000010.1).
• Konjugation: Unconjugated
• Alternative Synonym: acetoacetyl Coenzyme A thiolase, THIL, T2, MAT, ACAT, acetyl-Coenzyme A acetyltransferase 1, ACAT1

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: batch dependent
• Molekulargewicht: Observed: 42kDa
• NCBI: 38
• UniProt: P24752
• Puffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Formulierung: Liquid

Anwendungsbeschreibung

• Application Verdünnung: Optimal dilutions for each application to be determined by the researcher.
• Anwendungsbeschreibung: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200

Bilder

Western blot analysis of extracts of various cell lines, using ACAT1 antibody (19-597) at 1:5000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.

Immunohistochemistry of paraffin-embedded human lung cancer using ACAT1 antibody (19-597) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded rat spleen using ACAT1 antibody (19-597) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse lung using ACAT1 antibody (19-597) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of C6 cells using ACAT1 antibody (19-597) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of NIH-3T3 cells using ACAT1 antibody (19-597) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of U-2 OS cells using ACAT1 antibody (19-597) at dilution of 1:100. Blue: DAPI for nuclear staining.