CIAPIN1 Antibody, Unconjugated, Rabbit, Polyclonal

Artikelnummer: PRS-22-138
Artikelname: CIAPIN1 Antibody, Unconjugated, Rabbit, Polyclonal
Artikelnummer: PRS-22-138
Hersteller Artikelnummer: 22-138
Alternativnummer: PRS-22-138-100
Hersteller: ProSci
Wirt: Rabbit
Kategorie: Antikörper
Applikation: IF, IHC, IP, WB
Spezies Reaktivität: Human, Mouse
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-312 of human CIAPIN1 (NP_064709.2).
Konjugation: Unconjugated
Alternative Synonym: Anamorsin {ECO:0000255|HAMAP-Rule:MF_03115}, Cytokine-induced apoptosis inhibitor 1 {ECO:0000255|HAMAP-Rule:MF_03115}, Fe-S cluster assembly protein DRE2 homolog {ECO:0000255|HAMAP-Rule:MF_03115}, CIAPIN1 {ECO:0000255|HAMAP-Rule:MF_03115}
Klonalität: Polyclonal
Konzentration: batch dependent
Molekulargewicht: Observed: 39kDa
NCBI: 57019
UniProt: Q6FI81
Puffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Formulierung: Liquid
Application Verdünnung: Optimal dilutions for each application to be determined by the researcher.
Anwendungsbeschreibung: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200IP: 1:50 - 1:100
Western blot analysis of extracts of various cell lines, using CIAPIN1 antibody (22-138) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.
Immunohistochemistry of paraffin-embedded human liver damage using CIAPIN1 Antibody (22-138) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human gastric cancer using CIAPIN1 Antibody (22-138) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human leiomyoma of uterus using CIAPIN1 Antibody (22-138) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using CIAPIN1 antibody (22-138). Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 200ug extracts of 293T cells, using 3 ug CIAPIN1 antibody (22-138). Western blot was performed from the immunoprecipitate using CIAPIN1 antibody (22-138) at a dilition of 1:1000.