AHSP Antibody, Unconjugated, Rabbit, Polyclonal

Artikelnummer: PRS-22-245
Artikelname: AHSP Antibody, Unconjugated, Rabbit, Polyclonal
Artikelnummer: PRS-22-245
Hersteller Artikelnummer: 22-245
Alternativnummer: PRS-22-245-100
Hersteller: ProSci
Wirt: Rabbit
Kategorie: Antikörper
Applikation: IHC, WB
Spezies Reaktivität: Human, Mouse, Rat
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-102 of human AHSP (NP_057717.1).
Konjugation: Unconjugated
Alternative Synonym: EDRF, ERAF, alpha-hemoglobin-stabilizing protein, alpha hemoglobin stabilising protein, erythroid differentiation associated factor, erythroid differentiation-related factor
Klonalität: Polyclonal
Konzentration: batch dependent
Molekulargewicht: Observed: 12kDa
NCBI: 51327
UniProt: Q9NZD4
Puffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Formulierung: Liquid
Application Verdünnung: Optimal dilutions for each application to be determined by the researcher.
Anwendungsbeschreibung: WB: 1:500 - 1:2000IHC: 1:50 - 1:200
Western blot analysis of extracts of various cell lines, using AHSP antibody (22-245) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 15s.
Immunohistochemistry of paraffin-embedded rat testis using AHSP antibody (22-245) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using AHSP antibody (22-245) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat heart using AHSP antibody (22-245) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human breast using AHSP antibody (22-245) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse kidney using AHSP antibody (22-245) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse heart using AHSP antibody (22-245) at dilution of 1:100 (40x lens).