HSPH1 Antibody, Unconjugated, Rabbit, Polyclonal

Artikelnummer: PRS-22-359
Artikelname: HSPH1 Antibody, Unconjugated, Rabbit, Polyclonal
Artikelnummer: PRS-22-359
Hersteller Artikelnummer: 22-359
Alternativnummer: PRS-22-359-100
Hersteller: ProSci
Wirt: Rabbit
Kategorie: Antikörper
Applikation: IHC, WB
Spezies Reaktivität: Human, Mouse, Rat
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 659-858 of human HSPH1 (NP_006635.2).
Konjugation: Unconjugated
Alternative Synonym: Heat shock protein 105 kDa, Antigen NY-CO-25, Heat shock 110 kDa protein, HSPH1, HSP105, HSP110, KIAA0201
Klonalität: Polyclonal
Konzentration: batch dependent
Molekulargewicht: Observed: 120kDa
NCBI: 10808
UniProt: Q92598
Puffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Formulierung: Liquid
Application Verdünnung: Optimal dilutions for each application to be determined by the researcher.
Anwendungsbeschreibung: WB: 1:500 - 1:2000IHC: 1:50 - 1:200
Western blot analysis of extracts of various cell lines, using HSPH1 antibody (22-359) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s.
Immunohistochemistry of paraffin-embedded rat spleen using HSPH1 antibody (22-359) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat fallopian tube using HSPH1 antibody (22-359) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human tonsil using HSPH1 antibody (22-359) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human vermiform appendix using HSPH1 antibody (22-359) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse spinal cord using HSPH1 antibody (22-359) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using HSPH1 antibody (22-359) at dilution of 1:100 (40x lens).