PNKP Antibody, Unconjugated, Rabbit, Polyclonal

Produktübersicht

• Artikelname: PNKP Antibody, Unconjugated, Rabbit, Polyclonal
• Artikelnummer: PRS-22-410
• Hersteller Artikelnummer: 22-410
• Alternativnummer: PRS-22-410-100
• Hersteller: ProSci
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: IF, IHC, WB
• Spezies Reaktivität: Human, Mouse, Rat
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 60-320 of human PNKP (NP_009185.2).
• Konjugation: Unconjugated
• Alternative Synonym: PNKP, polynucleotide kinase 3-phosphatase, PNK, polynucleotide kinase 3-prime-phosphatase, MCSZ, EIEE10

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: batch dependent
• Molekulargewicht: Observed: 57kDa
• NCBI: 11284
• UniProt: Q96T60
• Puffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Formulierung: Liquid

Anwendungsbeschreibung

• Application Verdünnung: Optimal dilutions for each application to be determined by the researcher.
• Anwendungsbeschreibung: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:100

Bilder

Western blot analysis of extracts of various cell lines, using PNKP antibody (22-410) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.

Immunohistochemistry of paraffin-embedded rat kidney using PNKP antibody (22-410) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human lung cancer using PNKP antibody (22-410) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human prostate cancer using PNKP antibody (22-410) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse liver using PNKP antibody (22-410) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse heart using PNKP antibody (22-410) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of GFP-RNF168 transgenic U2OS cells using PNKP antibody (22-410). Green:GFP-RNF168 fusion protein expression for DNA damage marker.Blue: DAPI for nuclear staining. RNF168(GFP) can be used to mark cells damaged by UV-A laser for they always gather around DNA damage region.