SRP19 Antibody, Unconjugated, Rabbit, Polyclonal

Artikelnummer: PRS-22-461
Artikelname: SRP19 Antibody, Unconjugated, Rabbit, Polyclonal
Artikelnummer: PRS-22-461
Hersteller Artikelnummer: 22-461
Alternativnummer: PRS-22-461-100
Hersteller: ProSci
Wirt: Rabbit
Kategorie: Antikörper
Applikation: IF, IHC, WB
Spezies Reaktivität: Human, Mouse, Rat
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-144 of human SRP19 (NP_003126.1).
Konjugation: Unconjugated
Alternative Synonym: SRP19,
Klonalität: Polyclonal
Konzentration: batch dependent
Molekulargewicht: Observed: 16kDa
NCBI: 6728
UniProt: P09132
Puffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Formulierung: Liquid
Application Verdünnung: Optimal dilutions for each application to be determined by the researcher.
Anwendungsbeschreibung: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:10 - 1:100
Western blot analysis of extracts of various cell lines, using SRP19 antibody (22-461) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat ovary using SRP19 antibody (22-461) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using SRP19 antibody (22-461) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat spleen using SRP19 antibody (22-461) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human colon carcinoma using SRP19 antibody (22-461) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human vermiform appendix using SRP19 antibody (22-461) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using SRP19 antibody (22-461) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of MCF7 cells using SRP19 antibody (22-461). Blue: DAPI for nuclear staining.