COPB2 Antibody, Unconjugated, Rabbit, Polyclonal

Produktübersicht

• Artikelname: COPB2 Antibody, Unconjugated, Rabbit, Polyclonal
• Artikelnummer: PRS-22-648
• Hersteller Artikelnummer: 22-648
• Alternativnummer: PRS-22-648-100
• Hersteller: ProSci
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: IF, IHC, WB
• Spezies Reaktivität: Human, Mouse, Rat
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 657-906 of human COPB2 (NP_004757.1).
• Konjugation: Unconjugated
• Alternative Synonym: Coatomer subunit beta, Beta-coat protein, Beta-COP, p102, COPB2

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: batch dependent
• Molekulargewicht: Observed: 102kDa
• NCBI: 9276
• UniProt: P35606
• Puffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Formulierung: Liquid

Anwendungsbeschreibung

• Application Verdünnung: Optimal dilutions for each application to be determined by the researcher.
• Anwendungsbeschreibung: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:100

Bilder

Western blot analysis of extracts of various cell lines, using COPB2 antibody (22-648) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 5s.

Immunohistochemistry of paraffin-embedded rat brain using COPB2 antibody (22-648) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded rat heart using COPB2 antibody (22-648) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse brain using COPB2 antibody (22-648) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse kidney using COPB2 antibody (22-648) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse heart using COPB2 antibody (22-648) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of HeLa cells using COPB2 antibody (22-648) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Immunofluorescence analysis of NIH/3T3 cells using COPB2 antibody (22-648) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Immunofluorescence analysis of PC-12 cells using COPB2 antibody (22-648) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.