EFTUD2 Antibody, Unconjugated, Rabbit, Polyclonal

Artikelnummer: PRS-22-651
Artikelname: EFTUD2 Antibody, Unconjugated, Rabbit, Polyclonal
Artikelnummer: PRS-22-651
Hersteller Artikelnummer: 22-651
Alternativnummer: PRS-22-651-100
Hersteller: ProSci
Wirt: Rabbit
Kategorie: Antikörper
Applikation: IF, IHC, IP, WB
Spezies Reaktivität: Human, Mouse, Rat
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-205 of human EFTUD2 (NP_004238.3).
Konjugation: Unconjugated
Alternative Synonym: 116 kDa U5 small nuclear ribonucleoprotein component, Elongation factor Tu GTP-binding domain-containing protein 2, SNU114 homolog, hSNU114, U5 snRNP-specific protein, 116 kDa, U5-116 kDa, EFTUD2, KIAA0031, SNRP116
Klonalität: Polyclonal
Konzentration: batch dependent
Molekulargewicht: Observed: 120kDa
NCBI: 9343
UniProt: Q15029
Puffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Formulierung: Liquid
Application Verdünnung: Optimal dilutions for each application to be determined by the researcher.
Anwendungsbeschreibung: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:100IP: 1:20 - 1:50
Western blot analysis of extracts of various cell lines, using EFTUD2 antibody (22-651) at 1:2000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 20s.
Immunohistochemistry of paraffin-embedded rat liver using EFTUD2 Antibody (22-651) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using EFTUD2 Antibody (22-651) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human gastric cancer using EFTUD2 Antibody (22-651) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using EFTUD2 Antibody (22-651) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse kidney using EFTUD2 Antibody (22-651) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using EFTUD2 antibody (22-651).
Immunoprecipitation analysis of 100ug extracts of 293T cells using 3ug EFTUD2 antibody (22-651). Western blot was performed from the immunoprecipitate using EFTUD2 antibody (22-651) at a dilition of 1:1000.