MDC1 Antibody, Unconjugated, Rabbit, Polyclonal

Artikelnummer: PRS-23-531
Artikelname: MDC1 Antibody, Unconjugated, Rabbit, Polyclonal
Artikelnummer: PRS-23-531
Hersteller Artikelnummer: 23-531
Alternativnummer: PRS-23-531-100
Hersteller: ProSci
Wirt: Rabbit
Kategorie: Antikörper
Applikation: IF, IHC, WB
Spezies Reaktivität: Human
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-350 of human MDC1 (NP_055456.2).
Konjugation: Unconjugated
Alternative Synonym: IER3, NFBD1, KIAA0170, DKFZp781A0122
Klonalität: Polyclonal
Konzentration: batch dependent
Molekulargewicht: Observed: 270kDa
NCBI: 9656
UniProt: Q14676
Puffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Formulierung: Liquid
Application Verdünnung: Optimal dilutions for each application to be determined by the researcher.
Anwendungsbeschreibung: WB: 1:500 - 1:2000IHC: 1:100 - 1:200IF: 1:50 - 1:200
Western blot analysis of extracts of various cell lines, using MDC1 antibody (23-531) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit._Exposure time: 5s.
Immunohistochemistry of paraffin-embedded human lung cancer using MDC1 antibody (23-531) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human colon carcinoma using MDC1 antibody (23-531) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human prostate using MDC1 antibody (23-531) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human prostate cancer using MDC1 antibody (23-531) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human esophagus using MDC1 antibody (23-531) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U-2 OS cells using MDC1 antibody (23-531) at dilution of 1:100. Blue: DAPI for nuclear staining.