IKK epsilon Antibody, Unconjugated, Rabbit, Polyclonal

Produktübersicht

• Artikelname: IKK epsilon Antibody, Unconjugated, Rabbit, Polyclonal
• Artikelnummer: PRS-2329
• Hersteller Artikelnummer: 2329
• Alternativnummer: PRS-2329-0.02,PRS-2329-0.1
• Hersteller: ProSci
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ELISA, IF, IHC-P, WB
• Spezies Reaktivität: Human, Mouse
• Immunogen: Anti-IKK epsilon antibody (2329) was raised against a peptide corresponding to 16 amino acids near the carboxy terminus of human IKK epsilon. The immunogen is located within the last 50 amino acids of IKK epsilon.
• Konjugation: Unconjugated
• Alternative Synonym: IKK epsilon Antibody: IKKE, IKKI, IKK-E, IKK-i, IKKE, KIAA0151, Inhibitor of nuclear factor kappa-B kinase subunit epsilon, Inducible I kappa-B kinase, I-kappa-B kinase epsilon

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 1 mg/ml
• Molekulargewicht: Predicted: 80kDObserved: 80 kD
• NCBI: 9641
• UniProt: Q14164
• Puffer: IKK epsilon Antibody is supplied in PBS containing 0.02% sodium azide.
• Formulierung: Liquid

Anwendungsbeschreibung

• Application Verdünnung: Optimal dilutions for each application to be determined by the researcher.
• Anwendungsbeschreibung: WB: 1-2 µg/mL, IHC: 10 µg/mL, IF: 20 µg/mL.Antibody validated: Western Blot in human and mouse samples, Immunohistochemistry in human samples, Immunofluorescence in human samples. All other applications and species not yet tested.

Bilder

Figure 4 Western Blot Validation in Jurkat Cell LysateLoading: 15 &956,g of lysates per lane.Antibodies: IKK epsilon 2329 (2 &956,g/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Figure 6 Immunofluorescence Validation of IKK epsilon in HeLa CellsImmunofluorescent analysis of 4% paraformaldehyde-fixed HeLa cells labeling IKK epsilon with 2329 at 20 &956,g/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).

Figure 5 Immunohistochemistry Validation of IKK epsilon in Human Pancreas Tissue Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-IKK epsilon antibody (2329) at 10 &956,g/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT, antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4&730,C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Figure 1 Induction Validation in Mouse Cell LineLoading: 15 &956,g of Raw264.7 cell lysates per lane.Antibodies: IKK epsilon 2329 (1 &956,g/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.Cells were treated with LPS (0.3 &956,g/mL) for 3 hrs (lane 2) and 6 hrs (lane 3) or with control (lane 1).

Figure 2 Western Blot Validation in Human and Mouse Cell LinesLoading: 15 &956,g of lysates per lane.Antibodies: IKK epsilon 2329 (1 &956,g/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Figure 3 Western Blot Validation in Mouse Tissue LysatesLoading: 15 &956,g of lysates per lane.Antibodies: IKK epsilon 2329 (2 &956,g/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.