Anti-ACE2 antibody (biotin) (3217) was raised against a peptide corresponding to 18 amino acids near the carboxy terminus of human ACE2. The immunogen is located within the last 50 amino acids of ACE2.
ACE2 Antibody is supplied in PBS containing 0.02% sodium azide.
Formulierung:
Liquid
Application Verdünnung:
Optimal dilutions for each application to be determined by the researcher.
Anwendungsbeschreibung:
IHC: 2 µg/mL Antibody validated: Immunohistochemistry in human samples. All other applications and species not yet tested.
Figure 1 ELISA Validation Coating Antigen: immunogen peptide, 3217P, 10 &956,g/mL, incubate at 4 &730,C overnight.Detection Antibodies: SARS-CoV-2 Spike antibody, 3217-biotin or 3217, dilution: 0.5-1000 ng/mL, incubate at RT for 1 hr. 3217-biotin was detected by HRP-conjugated streptavidin at 1:5,000 and 3217 was detected by anti-rabbit HRP conjugated secondary antibodies at 1:10,000 , incubate at RT for 1 hr.
Figure 2 Immunohistochemistry Validation of ACE-2 in COVID-19 Patient Lung Immunohistochemical analysis of paraffin-embedded human testis tissue using anti- ACE2 antibody (3217-biotin, 5 &956,g/mL). Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT, antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4&730,C, following by streptavidin-HRP conjugate at 20 &956,g/mL. Counter stained with Hematoxylin.
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